Content of malondialdehyde and activity of enzyme glutathione- S-transferase in the leaves of emmer wheat under the action of herbicide and plant growth regulator

Abstract

The article presents the results of vegetation experiment on the influence of different rates of herbicide Prima Forte 195 – 2-ethylhexyl ether 2.4-D + aminopyralid + florasulam (0.5; 0.6 and 0.7 L ha-1) under different application methods of plant growth regulator of a natural origin Wuxal BIO Vita (Ascophyllum nodosum extract + microelements) on the content of malondialdehyde (MDA) as an important indicator of the intensity of peroxide oxidation of lipids in plants and on the activity of enzyme glutathione-S-transferase (GST) in the leaves of emmer wheat. Experimental scheme included 16 experimental variants: 1 – without application of preparations and pre-sowing treatment of seeds (control); 2, 3, 4 – Prima Forte 195, applied to vegetative plants at the rates 0.5, 0.6 and 0.7 L ha-1; 5 – Wuxal BIO Vita at the rate of 1.0 L ha-1, applied to vegetative plants; 6, 7, 8 – Prima Forte 195 at the rates of 0.5, 0.6 and 0.7 L ha-1 in tank mixtures with Wuxal BIO Vita at the rate of 1.0 L ha-1, sprayed on vegetative plants; 9 – pre-sowing treatment of seeds with Wuxal BIO Vita at the rate of 1.0 L t-1 (background); 10, 11, 12 – Prima forte 195, applied to vegetative plants at the rates of 0.5, 0.6 and 0.7 L ha-1 at the background of pre-sowing treatment of seeds with Wuxal BIO Vita at the rate of 1.0 L t-1; 13 – Wuxal BIO Vita at the rate of 1.0 L ha-1 (applied to vegetative plants, treated before sowing with Wuxal BIO Vita at the rate of 1.0 L t-1); 14, 15, 16 – Prima Forte 195 at the rates of 0.5, 0.6 and 0.7 L ha-1 respectively, in tank mixture with Wuxal BІО Vita at the rate of 1.0 L ha -1 – spraying of plants and pre-sowing treatment of seeds with Wuxal BІО Vita 1.0 L t-1. It has been found that under the application of Prima Forte 195 the redox state in the leaves of emmer wheat increased considerably in the direction of increasing the content of MDA. It has also been proved that herbicide Prima Forte 195 and its mixtures with plant growth regulator (PGR) Wuxal BIO Vita caused the changes in the activity of enzyme glutathione-S-transferase, which slightly increased in the initial period in the variants of integrated application of herbicide and plant growth regulator. However, later the GST activity in the variants of integrated application of herbicide and PGR decreased, which indicates stabilization and a positive influence of integrated application of preparations on the detoxification processes in the crops of emmer wheat.