Influence of biologically active substances on key indicators of the conditions of winter wheat ecocenosis

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Дата
2021
Автори
Zabolotnyi, Oleksandr
Rozborska, Larysa
Leontiuk, Iryna
Zhilyak, Ivan
Datsenko, Anna
Назва журналу
ISSN журналу
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Видавець
Web of Conferences
Інструкція
The article deals with the study of the effect of the application of the herbicide Granstar Gold 75, w.g. (water-soluble granulate) and plant growth regulator Regoplant on some ecological indicators of ecocenosis of winter wheat sowing (lipid peroxidation reactions in winter wheat plants by the activity of malonic dialdehyde content accumulation, enzymatic activity, total number of rhizosphere bacteria and anatomical structure of winter wheat leaves). The choice of research topic is due to the fact that currently obtaining high yields of winter wheat is closely connected to the widespread use of chemicals, in particular, herbicides, which by their nature are physiologically active substances that can affect both plants and soil microbiota. This, in turn, leads to the search for environmentally safe elements of technologies for growing winter wheat. One such element may be the use of herbicides together with plant growth regulators. The obtained experimental data testify to the protective ability of the growth regulator Regoplant against the winter wheat plants, as evidenced by a decrease in the activity of malonic dialdehyde accumulation, changes in the activity of the enzymatic defense system, decrease the number of epidermis cells and an increase in total rhizosphere bacteria in case of Regoplant use together with Granstar Gold 75, w.g. compared with the experiment variants, where the herbicide was applied without a growth regulator. That is, the use of growth regulator in a mixture with herbicide to some extent eliminates the toxic effect of xenobiotics, which has a positive effect on the state of ecobiosis of winter wheat sowing.
Опис
Therefore, the obtained experimental data indicate that the use of herbicide Granstar Gold 75, w.g. and plant growth regulator Regoplant has a certain influence on such indicators of ecological stability of ecobiosis of winter wheat plantings as the intensity of lipid peroxidation reactions in the plants, the activity of enzymes and the total number of rhizosphere bacteria in the soil. The application of Granstar Gold 75, w.g. without plant growth regulator is characterized by a certain toxic effect on both winter wheat plants and soil microbiota, which is manifested in increased activity of accumulation of malonic dialdehyde (indicating the activation of the lipid peroxidation reactions), changes in the antioxidant enzymes activity and a certain decrease in the total number of rhizosphere bacteria. The degree of xenobiotic influence on the specified indicators of a condition of ecobiocis of winter wheat plantings has a tendency to strengthening together with the increase of the preparation use rate. So, in particular, on the third day after the application of Granstar Gold 75, w.g. in the rates of 15, 20, 25 and 30 g/ha without Regoplant, there was an increase in the intensity of LP reactions in winter wheat plants against control from 3.2 to 17.4 μMol MDA/g of raw substance, depending on the rate of xenobiotic application. The activity of the enzymatic defense system increased in 1.18–1.46 times, depending on the rate of herbicide and type of enzyme. The total number of rhizosphere bacteria under the action of the herbicide without a growth regulator decreased from 1680 to 1510 thousand CFU by increasing the rate of the drug from 15 to 30 g/ha. The combined use of preparations indicates the protective effect of the growth regulator on winter wheat plants and soil microbiota, which is manifested in a certain slowdown in the reactions of lipid peroxidation and an increase in the number of rhizosphere bacteria. In particular, on the fifth day after the application, the accumulation of malonic dialdehyde, although exceeding the control variant, but against the variants of the experiment with the use of herbicide without Regoplant decreased by 1.7–7.1 μMol MDA/g of raw substance according to the herbicide rates in a mixture with plant growth regulator. Under the joint application of preparations by 40–70 thousand CFU, the number of rhizosphere bacteria increased compared to the use of herbicide without plant growth regulator. Also under the using of preparationі was noted dependence to decrease the number of epidermis cells of winter wheat leaves with a simultaneous increase in their surface.
Ключові слова
winter wheat, Granstar Gold, Regoplant, LP reactions, activity of enzymes, rhizosphere bacteria, key indicators, anatomical structure, wheat ecocenosis
Бібліографічний опис
Oleksandr Zabolotnyi, Larysa Rozborska, Iryna Leontiuk, Ivan Zhilyak and Anna Datsenko. Influence of Biologically Active Substances on Key Indicators of the Conditions of Winter Wheat Ecocenosis. IV International Scientific Congress “Society of Ambient Intelligence – 2021” (ISCSAI 2021). Volume 100, 2021.